This study reports the prevalence of thyroid diseases and their relationship to autoimmunity in a population of Khulna district where goitre is not endemic. A survey was performed among citizens of a union of Khulna district. The study population consisted of a random sample of the students of primary schools, secondary schools and people of nearby areas. History taking, neck examination for goitre and blood examination for thyroid hormones was done from each subject. Blood sample was measured for thyroid hormones and thyroid microsomal antibody (TMAb) using radioimmunoassay method. Of the total 925 individual studied, 527 was female and 398 was male with age ranges from 2-62 years (mean 19.86::!:13.62 years).