Background: Brucellosis is an important re-emerging zoonosis which remains an uncontrolled public health problem worldwide because of serious diminution of manpower, animals and essential foodstuffs. Transmission to human can occurs through contact with infected animals or animal products. It is a poorly recognised health problem in this country though there is persistence of large reservoirs of the organism among livestock such as sheep, goat and cows creating fertile field for unabated transmission. But data on it’s transmission potential and prevalence in this country specially in Sylhet region remained scarce. In the absence of supportive epidemiological information physicians on most of the cases have to depend on their clinical judgement to suggest relevant tests.