Bangladesh Medical Research Council (BMRC)
Immunoreactivity with monoclonal antibodies against epithelial membrane antigen, vimentin, keratin-squamous epithelium, keratin-nonsquamous epithelium and with polyclonal antibodies against keratin, involucrin, 5-100 protein, desmin and x-1 antitrypsin was done in 30 pleural and peritoneal effusion fluids; 15 each of benign and malignant origin using the avidin-biotin peroxidase complex (ABC) technique to differentiate between the mesothelial cells and the adenocarcinoma cells. In the present study we have demonstrated that desmin and 5-100 protein are distributed in the cancer cells and the mesothelial cells of the effusion fluids. Neither EMAnor keratin has the specific reactive pattern which could…