Abstract: Objective: The objective of the study was to find out the reliability of Color Doppler Ultrasonography (CDUS) in the diagnosis of testicular lesions. Methodology: This crosssectional type of study was carried out in the department of Radiology & Imaging of Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh to determine the value of Color Doppler Ultrasonography (CDUS) as a routine investigation method for diagnosis of testicular lesions. For this purpose, a total of 51 clinically suspected patients of testicular complains were included in this study with age range from 18 to 65 yrs. Color Doppler Ultrasonography (CDUS) was done in all these patients. Vascularity of the lesions, PSV (Peak Systolic Velocity), RI (Resistivity Index) were doppler variable which showed increased vascularity and PSV with decreased RI in maximum testicular lesions. They were followed up for the Final Clinical Diagnosis based on response to the medical treatment, Fine needle aspiration cytology (FNAC), histopathology and operative findings. Result: The reliability of CDUS was shown by kappa value which was 0.797 with a p value of <0.05. This measure of agreement was statistically significant with substantial agreement between CDUS & Final Diagnosis of