Bangladesh Medical Research Council (BMRC)
Celt monolayers grown in polystyrene culture plates and in glass tubes are routinely used in virology laboratorise around the world for virus isolation and identification. Monolayers of different types of cells grown in polystyrene culture plates were found to show a strange cytotoxicity when inoculated with stool samples rrom healthy children from Bangladesh. When inoculated with 0.2 ml of a .10% stool sample 100% (80/80) of the cell monolayers eShblished in polystyrene plates showod cytopathy by 4 days…