Background: A unique challenge for clinical microbiologists, clinicians, infection control professionals is to deal with extended-spectrum β-lactamase (ESBL) producing pathogens. The study aims to isolate ESBL producing bacteria from urine samples of Urinary Tract Infection (UTI)-patients and to analyze their phenotypic and genotypic characteristics.
Materials and Methods: A total of 90 urine samples from UTI patients were collected from Enam Medical College Hospital and Gonoshastha Medical College Hospital, Savar, Dhaka, Bangladesh; between May-2012 to August-2012. A total 75 gram negative isolates were retrieved and screened for ESBL production by the Double Disk Diffusion Synergy Test (DDST). Isolates with ESBL phenotype were further characterized by antibiotic susceptibility testing, PCR and sequencing of β-lactamase genes.