Bangladesh Medical Research Council (BMRC)
During the course of a study on thermal inactivation of cholera phages (Monsur et aI., 1975) we observed that the results at the higher temperature range (65°C to 700C and above) were erratic. For example, if a tube containing a known dilution of the phage was heated in a water bath and the contents examined at intervals for the number of plaque forming units (p.f.u.) present, it was not unusual for the later samples to give a higer count than the earlier ones; sometimes duplicate samples removed from the tube at…